A number of features of the 5′ UTR upstream of the human p36 open reading frame of BAG 1 suggested that it would be involved in the translational regulation of BAG 1. For example, it is long (410 nucleotides) and G C rich and has alternative translation initiation start codons including an upstream non canonical CUG. To investigate whether the p36 BAG 1 5′ UTR could contain an internal ribosome entry segment, a dicistronic reporter assay was used.
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